By Laura Dean, National Center for Biotechnology Information (U.S.), National Center for Biotechnology Information (U.S.).
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This condition is usually fatal, either in utero or soon after birth. The Coombs test detects Rh incompatibility between mother and fetus To detect HDN, the presence of maternal anti-Rh IgG must be identified. In vivo, these antibodies destroy Rh D-positive fetal RBCs, but in vitro, they do not lyse cells or even cause agglutination, making them difficult to identify. Therefore, the Coombs test is used. This test uses antibodies that bind to anti-D antibodies. The test is named for Robin Coombs, who first developed the technique of using antibodies that are targeted against other antibodies.
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Chapter 7. The Rh blood group Page 5 of 6 References 1. Reid ME and Lomas-Francis C. The Blood Group Antigen Facts Book. Second ed. 2004, New York: Elsevier Academic Press. 2. Avent ND, Reid ME. The Rh blood group system: a review. Blood 2000; 95:375-87. 3. Daniels G. The molecular genetics of blood group polymorphism. Transpl Immunol 2005; 14(3-4):143153. 4. Westhoff CM. The Rh blood group system in review: a new face for the next decade. Transfusion 2004; 44:1663-73. 5. Daniels GL. Human Blood Groups.