Renal and Urinary Proteomics: Methods and Protocols by Thongboonkerd

By Thongboonkerd

In line with learn and effects from the foreign Human Kidney and Urine Proteome undertaking, this instruction manual and reference provides present equipment and protocols for the research of human kidney and urine tract. The world's major specialists disguise every thing from easy suggestions for regimen research to complicated equipment for tough samples or analytes. Written with the practitioner in brain, the entire tools and strategies are defined with step by step protocols subsidized via beneficial functional recommendation taken from actual lifestyles experiments.

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2) as it is simple and practical. Human glomeruli are purified by using #40-, #80-, and #100-mesh sieves, respectively, as for the bovine glomeruli [7]. Although the purity of the glomeruli isolated by the sieving method is relatively high from large animals such as cows or humans, it is not so high enough from small animals such as rats, mice, or rabbits for precise studies on the glomerulus. 1 Standard sieving method. 5 6 1 Isolation and Enrichment of Glomeruli Using Sieving Techniques The numbers of sieving mesh in triple sets for glomerular isolation from several mammals.

The supernatant was removed gently using a Pasteur pipette. The pellet resuspended in the solution was rich in the glomeruli and a small drop of the suspension put on a slide was examined for the purity of the glomeruli under a phase-contrast microscope. This technique was modified and applied also for isolation of glomeruli from other mammals [5]. 3 Methods and Protocols were pressed through a stainless mesh sieve of #60 (pore size: 250 µm) with a spatula and rinsed with culture medium, and the filtered material was poured on a #300 mesh (pore size: 53 µm).

37. 38. 39. 40. dehydrogenase and its osmotic regulation in inner medullary collecting duct cells. Cell Physiol Biochem, 8, 293–303. D. B. (1990) In vivo osmoregulation of aldose reductase mRNA, protein, and sorbitol in renal medulla. Am J Physiol, 258, F154–F161. L. , and Garcia-Perez, A. (1992) Kidney aldose reductase gene transcription is osmotically regulated. Am J Physiol, 262, C776–C782. D. Jr et al. (1989) Molecular cloning of cDNA coding for kidney aldose reductase. Regulation of specific mRNA accumulation by NaCl-mediated osmotic stress.

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