By Roger A. Freeman
Camouflage & Markings quantity 17: Consolidated B-24 Liberator U.S.A.A.F., E.T.O. & M.T.O., 1942-1945 КНИГИ ;ВОЕННАЯ ИСТОРИЯ Название: Camouflage & Markings quantity 17: Consolidated B-24 Liberator U.S.A.A.F., E.T.O. & M.T.O., 1942-1945Автор: Roger A. FreemanИздательство: Ducimus BooksГод: 1971Страниц: 24Формат: PDF в RARРазмер: 8.13МБЯзык: английскийB/w images, illustrations, colour illustrations, paint schemes, choosing marks.Скачать: DepositfilesUploading Hotfile eighty five
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32 2 Cyclization of 2,3-Oxidosqualene – The First Committed Step in Triterpenoid Biosynthesis . . . . . . . . . . . 1 Resolution of Cyclase Activities Required for Sterol and Triterpenoid Biosynthesis . . . . . . . . Cloning of 2,3-Oxidosqualene Cyclases . . . . . OSCs Required for Sterol Biosynthesis . . . . . . Triterpenoid Cyclases . . . . . . . . . . OSC Gene Families . . . . . . . . . . . The Relationship Between Structure and Function . .
Cerevisiae are not feasible because of the lack of appropriate mutants. However, LS-deficient yeast mutants accumulate high levels of 2,3-oxidosqualene, favouring the synthesis of novel cyclisation products generated by heterologous expression of OSCs. The absence of lanosterol also facilitates analysis of the reaction products. Corey and co-workers isolated a cDNA encoding Arabidopsis thaliana CS by transforming a plant cDNA expression library into such a yeast mutant and screening protein preparations derived from pools of transformants for the ability to synthesise cycloartenol by TLC .
Biosynthesis will facilitate the development of plants with altered saponin content. In some cases enhanced levels of saponins or the synthesis of novel saponins may be desirable (for example, for drug production [9, 12–15] or improved disease resistance [4, 6, 10, 16]) while for other plants reduction in the content of undesirable saponins would be beneficial (for example, for legume saponins that are associated with antifeedant properties in animal feed ). This review is concerned with recent progress that has been made in the characterisation of the enzymes and genes involved in the synthesis of these complex molecules, and focuses on triterpenoid saponins.