Neuroanatomical Research Techniques by Richard T. Robertson

By Richard T. Robertson

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Terminal degeneration within the central nervous system as studied by a new silver method. Journal of Neuropathology and Experimental Neurology, 1946, 5 , 54-59. Gray, P. ) The encyclopedia of microscopy and microtechnique. Princeton, New Jersey: Van Nostrand-Reinhold, 1973. , Groat, R. , and Windle, W. F. A physiological approach to perfusion-fixation of tissues with formalin. Stain Technology, 1945, 2 0 , 13-22. Lillie, R. , Welsh, R. , Holmquist, N. , Donaldson, P. , & Berger, C. A 2. BASIC HISTOLOGICAL TECHNIQUES 45 consideration of substitutes for alum hematoxylin in routine histologic and cytologic diagnostic procedures.

Their obvious advantage is one of increasing the ease and speed of data entry. A second type of semiautomatic system falls just short of being a truly automatic system. This class of systems is able to process the specimen image and extract the pertinent structural features mostly without operator intervention. However, concessions to the complexity of the image must be made, and the operator is usually required to monitor the computer's performance, aiding it where it is unable to completely delineate the desired structure.

Methodology A. Delineation of Components B. Three Specific Systems IV. Appearance of the Data, Analysis, and Interpretation A. Examples B. Other Applications C. Computer Analyses of Quantized Data V. Technical Problems, Methodological Limitations, and Interpretive Difficulties A. Specimens B. Hardware and Software C. Technical Assistance D. Investigator Responsibility VI. Summary and Comments References 48 49 50 50 52 3 53 63 68 68 77 78 79 79 80 81 81 81 83 5 47 Copyright © 1978 by Academic Press, Inc.

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