By Waldo E. Cohn, Kivie Moldave (Eds.)
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Subtilis but are different from E . coli, providing further evidence that mycoplasmas are related to gram-positive bacteria. The total sequence of 1 6 3 rRNA gene in rrnB of M . capricolum (28)is 1521 b p long, being 21 bp shorter than that of the E . coli 1 6 3 rRNA gene (39), with a remarkable sequence similarity to those of E . coli (74%)and A. The similarity to the B. The 1 6 4 rRNA gene of Mycoplasma strain PG50 is 99% identical with the M . capricolum sequence (22). Secondary structure models of eubacterial 16-S rRNA have been proposed (42-44).
Coli, this clone appears to contain the gene for guanylyltransferase. The insert contained an open reading frame encoding a polypeptide of 459 amino acids with a calculated M , of 52,764, which corresponds to the size for the intact a subunit (52 LDA). The identity of the gene was further confirmed by expressing the gene in E . guanyl yltrans ferase. From Northern blot analysis4 (82) of the yeast poly(A)+-RNAusing a fragment containing the open reading frame as a probe, the size of the guanylyltransferase mRNA was estimated to be about 1600 bases.