Research in Protozoology by Tze-Tuan ed. CHEN

By Tze-Tuan ed. CHEN

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E. 0. Their work indicates that not only the protein, but also the non-protein amino acid composition is rather carefully regulated in these cells. Evidence of genetic control of amino acid composition has been obtained in another line of research. Studying T. pyriformis, variety 6, mating types I and II, Wells (248) established differences between the two types in the ratio of free arginine to alanine, and in free isoleucine to leucine. A progeny clone, derived from conjugation of the two types, resembled the one parent with respect to the one ratio and the other in regard to the second.

It was found by Cerroni and Zeuthen (38) that macronuclei engaged asynchronously in DNA synthesis for some time after EH, and their observation has been confirmed in later experiments which indicate that this interval lasts for a period of between 20 and 55 minutes after EH (Fig. 3 of Hjelm and Zeuthen (103) ). From this time (60-30 minutes prior to the first synchronous division maximum) onwards there was decreasing participation in DNA synthesis, and around EH + 75-80 minutes extremely few macronuclei were engaged in DNA synthesis.

This hypothesis stated that division-synchrony could be obtained, if the asynchron- SYNCHRONIZED CELL DIVISION IN PROTOZOA 51 ously dividing cells could be differentially affected merely in their progression through the cell cycle. (237) These regressions and progressions phase the cells for morphogenesis and for cell division, two closely coupled phenomena in this organism. While this case is mathematically manageable, (36) it is still too simple to account for the additional experimental finding that cortical structures regress while at the same time macronuclear chemical events progress within the same cell.

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